basic nucleofector kit primary mammalian endothelial cells Search Results


amaxatm basic nucleofectortm kit for primary mammalian endothelial cells  (Lonza)
90
Lonza amaxatm basic nucleofectortm kit for primary mammalian endothelial cells
CTEPH plasma shows increased levels of endothelium-derived microparticles compared with pulmonary embolic and healthy controls. a Scanning electron microscope images of MP fractions.from a healthy volunteer, CTEPH and PE patients. Bar = 2 μm. b Number of membrane (PKH26 + ) MPs and c number of VE-cadherin + (CD144 + ) MPs in plasma of healthy voulnteers, CTEPH and PE patients, as indicated; d Endoglin levels in MP fractions; ELISA assay; e <t>endothelial</t> angiogenesis (Matrigel tube formation) in HPAECs cultured with MPs isolated from CTEPH, PE or healthy plasma, as indicated. ** P < 0.01; * P < 0.05, comparisons with healthy controls, ## P < 0.01; # P < 0.05, comparisons with CTEPH; N = 4-6. Representative images of tube formation are shown beside the graph. Bar = 50 μm
Amaxatm Basic Nucleofectortm Kit For Primary Mammalian Endothelial Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CTEPH plasma shows increased levels of endothelium-derived microparticles compared with pulmonary embolic and healthy controls. a Scanning electron microscope images of MP fractions.from a healthy volunteer, CTEPH and PE patients. Bar = 2 μm. b Number of membrane (PKH26 + ) MPs and c number of VE-cadherin + (CD144 + ) MPs in plasma of healthy voulnteers, CTEPH and PE patients, as indicated; d Endoglin levels in MP fractions; ELISA assay; e endothelial angiogenesis (Matrigel tube formation) in HPAECs cultured with MPs isolated from CTEPH, PE or healthy plasma, as indicated. ** P < 0.01; * P < 0.05, comparisons with healthy controls, ## P < 0.01; # P < 0.05, comparisons with CTEPH; N = 4-6. Representative images of tube formation are shown beside the graph. Bar = 50 μm

Journal: Journal of Biomedical Science

Article Title: Endothelium-derived microparticles from chronically thromboembolic pulmonary hypertensive patients facilitate endothelial angiogenesis

doi: 10.1186/s12929-016-0224-9

Figure Lengend Snippet: CTEPH plasma shows increased levels of endothelium-derived microparticles compared with pulmonary embolic and healthy controls. a Scanning electron microscope images of MP fractions.from a healthy volunteer, CTEPH and PE patients. Bar = 2 μm. b Number of membrane (PKH26 + ) MPs and c number of VE-cadherin + (CD144 + ) MPs in plasma of healthy voulnteers, CTEPH and PE patients, as indicated; d Endoglin levels in MP fractions; ELISA assay; e endothelial angiogenesis (Matrigel tube formation) in HPAECs cultured with MPs isolated from CTEPH, PE or healthy plasma, as indicated. ** P < 0.01; * P < 0.05, comparisons with healthy controls, ## P < 0.01; # P < 0.05, comparisons with CTEPH; N = 4-6. Representative images of tube formation are shown beside the graph. Bar = 50 μm

Article Snippet: HPAECs were transfected using AmaxaTM Basic NucleofectorTM Kit for Primary Mammalian Endothelial Cells (Lonza, Switzerland) applying optimal NucleofectorTM program M-003.

Techniques: Clinical Proteomics, Derivative Assay, Microscopy, Membrane, Enzyme-linked Immunosorbent Assay, Cell Culture, Isolation

MPs obtained from endoglin-overexpressing HPAECs stimulate endothelial cell survival and angiogenesis in vitro . a Internalisation of fluorescently-labelled MPs by HPAECs (2 h incubation). In the merged image, PKH26 is red, HA is blue and endoglin is green, as indicated. White pixels mark co-localization of PKH26, HA and endoglin and indicate intracellular localization of microparticles carrying recombinant endoglin (Image J analysis). Bar = 2 μm. b Cell proliferation, ( c ) cell viability and ( d ) angiogenesis/tube formation in HPAECs treated with MPs isolated from untreated (control) HPAECs, cells transfected with empty pDisplay vector and HPAECs overexpressing pDisplay-L- endoglin, as indicated. e Representative images of tube formation in cells transfected with pDisplay or pDisplay-L-endoglin.* P < 0.05; *** P < 0.001, comparison with transfection controls; Bar = 50 μm; n = 3

Journal: Journal of Biomedical Science

Article Title: Endothelium-derived microparticles from chronically thromboembolic pulmonary hypertensive patients facilitate endothelial angiogenesis

doi: 10.1186/s12929-016-0224-9

Figure Lengend Snippet: MPs obtained from endoglin-overexpressing HPAECs stimulate endothelial cell survival and angiogenesis in vitro . a Internalisation of fluorescently-labelled MPs by HPAECs (2 h incubation). In the merged image, PKH26 is red, HA is blue and endoglin is green, as indicated. White pixels mark co-localization of PKH26, HA and endoglin and indicate intracellular localization of microparticles carrying recombinant endoglin (Image J analysis). Bar = 2 μm. b Cell proliferation, ( c ) cell viability and ( d ) angiogenesis/tube formation in HPAECs treated with MPs isolated from untreated (control) HPAECs, cells transfected with empty pDisplay vector and HPAECs overexpressing pDisplay-L- endoglin, as indicated. e Representative images of tube formation in cells transfected with pDisplay or pDisplay-L-endoglin.* P < 0.05; *** P < 0.001, comparison with transfection controls; Bar = 50 μm; n = 3

Article Snippet: HPAECs were transfected using AmaxaTM Basic NucleofectorTM Kit for Primary Mammalian Endothelial Cells (Lonza, Switzerland) applying optimal NucleofectorTM program M-003.

Techniques: In Vitro, Incubation, Recombinant, Isolation, Control, Transfection, Plasmid Preparation, Comparison